Coding

Part:BBa_K2271017:Design

Designed by: Fiona Edenhofer, Kristin Gehling   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)


VioE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1561
    Illegal BglII site found at 880
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1916
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promotor (ScPGK1; strong) and terminator (ScADH1) are optimized for yeast, whereas the coding sequence is not. A 6X-His/3X-Flag-Tag is added to the N-terminus.

Source

Amplification via PCR from biobrick VioABCE (Part:BBa_K274004)

References